We investigated the training effects of mental rotation of two-dimensional figures. 35 participants practiced rotating the upright image into the orientation between 0° to 180° with a set of 4 figures. Then they were tested on the mental rotation into the new orientation between 0° to 180° with the same set of figures (new orientation condition), on the rotation of the upside-down image into the orientation between 180° to 360° with the old figures (novel orientation condition), and on the rotation with the different set of figures (novel figure condition). There was no difference in rotating rate between the novel orientation condition and the novel figure condition. The rotating rate for these conditions was significantly lower than that for the new orientation condition. The results suggest that the training effects do not transfer beyond the path through which participants rotated images in the practice.